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Experimental infection of dogs (Canis
familiaris) with sporulated oocysts of Neospora caninum
Excerpts from the above linked document below: |
Three 8-weeks-old dogs (A, B and C) were
experimentally infected with brain of seropositive
domestic buffaloes (Bubalus
bubalis) examined for the presence of
antibodies anti-N. caninum by indirect fluorescent
antibody test (IFAT). |
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A group of three gerbils (Meriones unguiculatus) was
used as control of the viability of the oocysts of N.
caninum. Each gerbil was orally inoculated with
1000 oocysts and followed for a period of 60 days.
After this period the gerbils were anesthetized
intraperitoneally with 0.015 ml of 2% xylazine (Kensol®,
Konig, Brazil) associated with 0.03 ml of ketamine 10%
(Vetaset®, Fort Dodge, Brazil) and intracardiac blood
was collected. After the collection, the
gerbils were euthanized with 0.2 ml of T61® (Intervet,
Brazil) and a section of the brain and heart was
collected for the detection of the parasite by real-time
PCR and immunohistochemistry (IHC). In
addition, samples of spleen, liver and skeletal muscles
were analyzed by IHC. Direct examination of brain
sections, using smear slides and coverslips, were
performed to detect tissue cysts of N. caninum. |
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"Dogs 1 and 2 were euthanized 180 days after
infection and were examined for the detection of N.
caninum in tissues" |
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On May 24, 1943, the extermination camp
at Auschwitz, Poland, receives a new doctor, 32-year-old
Josef Mengele, a man who will earn the nickname
“the Angel of Death.”
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Dr. Mengele tortured and abused live
prisoners with injections of everything from petrol to
chloroform among his many horrors. |
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He also had a penchant for studying twins,
whom he would dissect. Born March 16, 1911, in Bavaria, Mengele
studied philosophy under
Alfred Rosenberg,
whose racial theories highly influenced him. In 1934, already a
member of the Nazi Party, he joined the research staff of the
Institute for Hereditary Biology and Racial Hygiene. |
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